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Influenza Virus Haemagglutinin: Estimation of Tryptophan and Tyrosine Content and Localization of Tryptophan Residues

The D.I. Ivanovsky Institute of Virology U.S.S.R. Academy of Medical Sciences, Gamaleya Str., 16, Moscow 123098, U.S.S.R.

The tryptophan and tyrosine content of the bromelain-released subtype H3 haemagglutinin (H3 BHA) of influenza virus were measured by u.v. absorption and fluorescence techniques. The values obtained (8 and 18 residues, respectively) are in close agreement with those derived from amino acid analysis. Essentially all of the tryptophan residues are demonstrated to be localized on the surface of the BHA molecule.

Received 25 September 1979; accepted 11 April 1980.