HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Puvion-Dutilleul, F. Articles by Lange, M. Search for Related Content PubMed PubMed Citation Articles by Puvion-Dutilleul, F. Articles by Lange, M. Agricola Articles by Puvion-Dutilleul, F. Articles by Lange, M.

In situ Study of SV40 Virus DNA in Lytic Infection by Mild Loosening of Nucleoproteins

¹ Groupe de Microscopie Electronique
² Groupe de Biophysique, Institut de Recherches Scientifiques sur le Cancer, CNRS, B.P. No. 8, F-94800 Villejuif, France

We have studied SV40 (simian virus 40) nucleoprotein in permissively infected monkey kidney cell cultures (CV1) by a procedure which does not require the isolation of the SV40 chromosomes. Treatment of the cells by a low ionic strength medium containing Photo flo produces a mild loosening of nucleoproteins, and permits the in situ study in ultrathin sections of virus components and their relationships with host cell chromatin. RNP and DNP could be distinguished by uranyl-EDTA-lead staining (for RNP) and by DNase digestion. SV40 DNA was observed as circular molecules, either free or connected with either RNP fibrils or virus capsids. These three aspects were interpreted, respectively, as viral minichromosomes, transcription of virus genome and partially encapsidated virus DNA. During encapsidation a few virus particles appear to be bound to host chromatin. Many, if not all, seemingly mature viruses, singly or in small linear clusters, are also aligned on host chromatin. Some of these observations were corroborated by the Miller spreading technique. They are consistent with a role for the host cell chromatin in the production of nuclear viruses.

^* To whom reprint requests should be addressed.

Received 25 January 1980; accepted 29 April 1980.

This article has been cited by other articles:

				F Puvion-Dutilleul, S Besse, E. Chan, E. Tan, and E Puvion p80-coilin: a component of coiled bodies and interchromatin granule-associated zones J. Cell Sci., January 3, 1995; 108(3): 1143 - 1153. [Abstract] [PDF]