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Resistance of Cucumber Protoplasts to Cucumber Mosaic Virus: a Comparative Study

Margaret I. Boulton

Department of Microbiology, University of Birmingham, Birmingham B15 2TT, U.K.

It was shown that resistance of the cucumber cultivar China (Kyoto) to cucumber mosaic virus (CMV) functions at the single cell level. Measurement of physiological parameters has excluded the possibility that this result arises from the artificial procedures used in protoplast isolation and inoculation. The maintenance of resistance in protoplasts of China (Kyoto) after inoculation with CMV RNA indicates that the resistance determinant(s) most probably operate at transcription or translation stages.

^* Present address: John Innes Institute, Colney Lane, Norwich NR4 7UH, U.K.

To whom reprint requests should be addressed.

Received 8 April 1980; accepted 23 June 1980.