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Hepatitis B Virus (HBV)-specific Structures found in Cytoplasmic Extracts of Cells producing HBV Surface Antigen (HBsAg) in vitro

¹ Kimron Veterinary Institute, Bet Dagan, Israel
and² Max-Planck-Institute of Biochemistry, Martinsried, West Germany

Two kinds of hepatitis B virus-specific particles are present in cytoplasmic extracts of hepatoma cells synthesizing hepatitis B virus (HBV) surface antigen. One class of particle contains the surface antigen of the virus, is 20S in size and has a buoyant density (in CsCl) of 1.2 g/ml. The second class of particle is a deoxyribonucleoprotein (DNP) with 1.3 g/ml buoyant density (in CsCl) and is 30S in size, the DNA of which contains HBV sequences thus proving virus specificity.

^* To whom correspondence should be addressed.

Received 28 May 1980; accepted 18 July 1980.