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J Gen Virol 51 (1980), 373-383; DOI 10.1099/0022-1317-51-2-373
© 1980 Society for General Microbiology
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Protein Synthesis in Cowpea Mosaic Virus-infected Cowpea Protoplasts: Further Characterization of Virus-related Protein Synthesis

Peter J. M. Rottier*, Geertje Rezelman and Ab Van Kammen

Department of Molecular Biology, Agricultural University, De Dreijen 11, 6703 BC Wageningen, The Netherlands

Inoculation of cowpea protoplasts with purified middle component of cowpea mosaic virus (CPMV) did not result in the production of newly induced virus-related proteins. In contrast, infection of protoplasts with purified bottom component produced virus-related polypeptides with mol. wt. 170x103, 112 x 103, 110 x 103, 84 x 103 and 30 x 103, but not the virus capsid proteins. These results suggest that CPMV B-RNA specifies one or more early functions, one of which is the replicase function, while M-RNA encodes the virus coat proteins. Attempts to elucidate the mechanism by which the virus-specific proteins are synthesized were inconclusive. No precursor-product relations could be demonstrated and virus subgenomic messengers were not observed. Some kind of processing was nonetheless found to occur with the small virus capsid protein:several discrete oligopeptides were successively cleaved from this protein after assembly into the virion particle.

* Present address: Institute of Virology, Veterinary Faculty, State University, Yalelaan 1, Utrecht, The Netherlands.

Received 19 February 1980; accepted 28 July 1980.




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