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Studies on the Culture, Assay of Infectivity and Some in vitro Properties of Lymphocystis Virus

B. J. Hill

Ministry of Agriculture, Fisheries and Food, Directorate of Fisheries Research, Fish Diseases Laboratory, Weymouth, Dorset DT4 8UB, U.K.

To facilitate an in vitro study of bluegill lymphocystis virus, a new cell line was established from the fins and caudal tissue of bluegill sunfish and designated BF-W. Bluegill lymphocystis virus was accurately and reproducibly assayed by an enlarged-cell enumeration assay and was propagated in BF-W cell cultures to give maximum titres of about 10⁶ cell-enlarging units/ml in 21 days at 25 °C. The infectivity titre of preparations of bluegill lymphocystis virus was not affected by ultrasonic treatment, freezing and thawing, or long-term storage in maintenance medium at -70 or +4 °C, but decreased during storage at -20 °C in 50% glycerol. The infectivity of virus preparations was reduced by treatment with diethyl ether. Virus cultured in BF-W cells was entirely cell-associated and the growth of virus was completely inhibited by 1 mM-5-BrdUrd.

^* Present address: Virology Department, Booth Hall Children's Hospital, Blackley, Manchester, U.K.

To whom reprint requests should be addressed.

Received 3 December 1979; accepted 30 July 1980.