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J Gen Virol 51 (1980), 421-423; DOI 10.1099/0022-1317-51-2-421
© 1980 Society for General Microbiology
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Physical Map of Tupaia Adenovirus DNA by Cleavage with Restriction Endonucleases and Partial Denaturation

Bertfried Matz1, Hajo Delius2, Rolf M. Flügel3 and Gholamreza Darai1

1 Institut für Medizinische Virologie der Universität Heidelberg, Im Neuenheimer Feld 324, 69 Heidelberg, West Germany
2 European Molecular Biology Laboratory, Meyerhofstrasse 1, 69 Heidelberg, West Germany
3 Institut für Virusforschung am Deutschen Krebsforschungszentrum, Im Neuenheimer Feld 280, 69 Heidelberg, West Germany

The double-stranded DNA of an adenovirus of tupaia (TAV) which has a mol. wt. of 21.5 x 106 was analysed as follows: the cleavage sites of restriction endonucleases BamHI, EcoRI, KpnI, SmaI, BstEII, ClaI and HpaI were determined by complete, partial and double digestion followed by gel electrophoretic separation of the resulting fragments. Terminal HpaI fragments were determined by hydrolysing the intact DNA with exonuclease III before restriction enzyme cleavage. Partial denaturation mapping of uncleaved DNA and EcoRI fragments revealed the cleavage sites of EcoRI as well as A+T-rich regions at both termini of the genome.

Received 21 April 1980; accepted 4 July 1980.


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E. Schondorf, U. Bahr, M. Handermann, and G. Darai
Characterization of the Complete Genome of the Tupaia (Tree Shrew) Adenovirus
J. Virol., April 1, 2003; 77(7): 4345 - 4356.
[Abstract] [Full Text] [PDF]


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