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Detection of Cytomegalovirus Genomes in Human Skin Fibroblasts by DNA Hybridization

¹ Department of Pediatrics, Children's Hospital, 700 Children's Drive, Columbus, Ohio 43205, U.S.A.
² Department of Veterinary Pathobiology The Ohio State University, Columbus, Ohio 43210, U.S.A.
³ Cancer Research Center and Department of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514, U.S.A.

A previous isolation of a human cytomegalovirus (CMV) from fibroblasts derived from intact skin of a Charcot-Marie-Tooth disease patient has prompted examination of six blind-coded cultured human skin lines by CMV DNA hybridization. The detection of CMV genome equivalents in three of the lines suggests that, in some cases, intact human skin may be a site of CMV latency.

^* On leave from the Institute of Microbiology, Medical University of Decebren, Decebren 4012, Hungary.

Received 2 May 1980; accepted 11 July 1980.