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Physical Mapping of Temperature-sensitive Mutations of Herpes Simplex Virus Type 2 by Marker Rescue

Pierre Chartrand

Morag C. Timbury

Medical Research Council Virology Unit, and Department of Virology, University of Glasgow, Glasgow G11 5JR, Scotland

The physical mapping of six ts mutations of herpes simplex virus type 2 (HSV-2) is presented. The results were obtained from 14 separate intratypic marker rescue experiments and the analysis of 20 HSV-1/HSV-2 intertypic recombinants. The order of these mutations on the physical map of HSV-2 is unambiguous and correlates almost exactly with the previously published genetic map of Timbury & Calder (1976). One of the mutants studied (HSV-2 ts 12) has apparently two distinct conditionally lethal ts mutations, one in the long and the other in the short region of the HSV genome.

Present address: Department of Microbiology, Faculty of Medicine, Centre Hospitalier Universitaire, Sherbrooke, Quebec, J1H 5N4, Canada.

Present address: Department of Bacteriology, Royal Infirmary, University of Glasgow, Glasgow G4 0SF, Scotland.

Received 28 April 1980; accepted 21 August 1980.