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Properties of the Virulent Form of a Mitomycin C- or Temperature-induced Thermophilic Bacteriophage

Department of Microbiology and Biochemistry Northwestern State University of Louisiana, Natchitoches, Louisiana 71457, U.S.A.

A virulent bacteriophage was isolated from lysates of Bacillus stearothermophilus strain NU-10 induced either by treatment with mitomycin C or by shifts in temperature. Optimum conditions for induction, morphology and other properties of the virus are described. Chloroform treatment and shifts in temperature of producer cells released approximately similar amounts of phage as did mitomycin induction, suggesting an effect on release rather than on synthesis of the virus.

Received 2 April 1980; accepted 16 July 1980.