HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Everett, R. D. Search for Related Content PubMed PubMed Citation Articles by Everett, R. D. Agricola Articles by Everett, R. D.

DNA Replication of Bacteriophage T5. 3. Studies on the Structure of Concatemeric T5 DNA

R. D. Everett

Microbiology Unit, Department of Biochemistry, South Parks Road, Oxford OX1 3QU, U.K.

The replication of bacteriophage T5 DNA has been shown to proceed via branched concatemeric intermediates. The structure of this concatemeric DNA was studied with respect to single-stranded regions and single-strand interruptions by digestion with S1 nuclease and agarose gel electrophoresis after alkali denaturation. The results were compared with the pattern of ‘nicks’ in the mature virion DNA, and the possible origins of these nicks are discussed. The structure of T5 concatemeric DNA was also studied by electron microscopy. Replication forks, loops and rare circular structures were observed, all of which were similar to those seen in replicating DNA of other large phages. Phage capsid structures were detected in association with both concatemeric and mature phage length DNA. These observations are discussed in relation to the replication, maturation and packaging of T5 DNA.

Present address: Department of Molecular Biology, King's Building, Mayfield Road, Edinburgh EH9 3JR, Scotland.

Received 9 June 1980; accepted 25 July 1980.

This article has been cited by other articles:

				L. Ponchon, P. Boulanger, G. Labesse, and L. Letellier The Endonuclease Domain of Bacteriophage Terminases Belongs to the Resolvase/Integrase/Ribonuclease H Superfamily: A BIOINFORMATICS ANALYSIS VALIDATED BY A FUNCTIONAL STUDY ON BACTERIOPHAGE T5 J. Biol. Chem., March 3, 2006; 281(9): 5829 - 5836. [Abstract] [Full Text] [PDF]