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J Gen Virol 52 (1981), 367-370; DOI 10.1099/0022-1317-52-2-367
© 1981 Society for General Microbiology

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The Location of the Bromelain Cleavage Site in a Hong Kong Influenza Virus Haemagglutinin

Theo A. Dopheide and Colin W. Ward

CSIRO, Division of Protein Chemistry, Parkville, 3052, Victoria, Australia

The site of bromelain cleavage in the haemagglutinin of the Hong Kong influenza virus A/Memphis/102/72 has been determined by using a diagonal peptide mapping procedure on the thermolytic digest of amidated BHA. The data show that bromelain cleavage removes the C-terminal 46 residues from HA2, and that the new carboxyl-terminal residue of BHA2 is Gly 175. This is close to the beginning of the hydrophobic membrane-interacting sequence that starts at residue 183.

Received 21 July 1980; accepted 3 September 1980.


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Q. Wang, X. Tian, X. Chen, and J. Ma
Structural basis for receptor specificity of influenza B virus hemagglutinin
PNAS, October 23, 2007; 104(43): 16874 - 16879.
[Abstract] [Full Text] [PDF]




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