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J Gen Virol 53 (1981), 125-132; DOI 10.1099/0022-1317-53-1-125
© 1981 Society for General Microbiology
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Electrophoretic Separation of Influenza Virus Ribonucleoproteins

P. J. Rees and N. J. Dimmock

Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, U.K.

A new procedure for the separation of influenza virus particle ribonucleoproteins (RNPs) by electrophoresis on a slab of polyacrylamide gel resolves five discrete bands. The largest is a triplet containing the three largest RNAs (1 to 3), the intermediate sized RNP is a doublet containing RNAs 5 and 6 and the others each contain a single RNA. In addition, each RNP is composed of NP protein and an amount of M which is independent of the size of the RNP. This suggests that some virus particle M is specifically associated with influenza ribonucleoprotein.

Received 5 August 1980; accepted 13 October 1980.


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