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Effects of Actinomycin D and Ultraviolet Irradiation on Multiplication of Brome Mosaic Virus in Host and Non-host Cells

¹ Laboratory of Plant Pathology Faculty of Agriculture, Kyoto University, Kyoto 606, Japan

T. Okuno²

² Laboratory of Plant Virology Department of Plant Science University of Alberta, Edmonton, Alberta, Canada

The modes of multiplication of brome mosaic virus (BMV) were compared in protoplasts isolated from host and non-host plants. BMV actively multiplied in the leaves and isolated mesophyll protoplasts of barley, a host of BMV. BMV multiplication in barley protoplasts was inhibited by addition of actinomycin D immediately after inoculation or by u.v. irradiation of the protoplasts before inoculation. In contrast, although BMV could not multiply in leaves of radish and turnip (non-hosts for BMV) it multiplied at a low level in protoplasts isolated from these two plant species. Moreover, u.v. irradiation, or the addition of actinomycin D, enhanced multiplication of BMV in radish and turnip protoplasts. These results suggest that (i) in the host cells replication of BMV is dependent on cellular metabolism of nucleic acid and protein, and (ii) in the non-host cells a substance(s) inhibitory to replication of BMV is synthesized.

Received 24 September 1980; accepted 27 November 1980.