HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Franklin, S. M. Articles by Epstein, M. A. Search for Related Content PubMed PubMed Citation Articles by Franklin, S. M. Articles by Epstein, M. A. Agricola Articles by Franklin, S. M. Articles by Epstein, M. A.

Antigenic Differences between the Membrane Antigen Polypeptides Determined by Different EB Virus Isolates

Department of Pathology University of Bristol Medical School, University Walk, Bristol BS8 1TD, U.K.

The antigenic specificities of the membrane antigen (MA) complex on three Epstein—Barr (EB) virus-producing cell lines have been compared by complete absorption of an anti-MA antiserum with P3HR-1 cells, followed by testing for residual anti-MA antibody activity against B95-8 and QIMR-WIL cells. Indirect membrane immunofluorescence showed that the absorbed serum, which had lost the capacity to bind P3HR-1 cells, nevertheless gave bright staining on a small proportion of B95-8 cells. SDS-polyacrylamide gel electrophoretic analysis of ¹²⁵I-labelled MA polypeptides showed that the absorbed serum was able to isolate MA polypeptides from both B95-8 and QIMR-WIL cells, but not from P3HR-1 cells. The results demonstrate that there is substantial sharing of antigenic specificities between the MA determined by three isolates of EB virus, and some antigenic divergence.

Received 20 August 1980; accepted 8 December 1980.