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J Gen Virol 54 (1981), 333-342; DOI 10.1099/0022-1317-54-2-333
© 1981 Society for General Microbiology
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Isolation and Characterization of a Temperature-sensitive Uncoating Mutant of Pseudorabies Virus

Larry Feldman{dagger}, Mayme L. Blankenship and Tamar Ben-Porat

Department of Microbiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37212, U.S.A.

During the course of characterizing a series of temperature-sensitive mutants of pseudorabies virus, we found one (designated tsL) that did not produce cytopathic changes in rabbit kidney cells at the non-permissive temperature (41 °C). Although the mutant adsorbed to and penetrated the cells in a normal fashion, virus RNA was not synthesized at 41 °C in the infected cells. However, if the cells were first incubated at the permissive temperature (32 °C), virus RNA synthesis occurred at the non-permissive temperature. This occurred even if, during the incubation period at 32 °C, the expression of viral functions was prevented by treatment with an inhibitor of protein synthesis. The DNA in tsL virions did not appear in the cell nucleus at 41 °C, and full, non-enveloped nucleocapsids could be recovered from the cytoplasm of tsL virus-infected cells. These results show that the nucleocapsids of tsL remain intact at the non-permissive temperature and that tsL is an uncoating mutant.

{dagger} Present address: The Rockefeller University, 1230 York Avenue, New York, N.Y. 10021, U.S.A.

Received 5 December 1980; accepted 23 February 1981.




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Copyright © 1981 by the Society for General Microbiology.