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The Nature of the DNA Associated with Incomplete Particles of Adenovirus Type 2

Govindranathsing Khittoo

Département de Microbiologie, Centre Hospitalier Universitaire, Université de Sherbrooke, Sherbrooke, Québec, Canada J1H 5N4

The nature of the DNA in incomplete particles (IP) synthesized by adenovirus type 2 and the ts4 mutant which accumulates such particles were analysed by agarose gel electrophoresis, restriction endonuclease cleavage and blot hybridization techniques. IP DNA consisted of a heterogeneous population of subgenomic-size DNA (IPSD₁) and smaller molecules ranging from about 1000 base pairs to 200 base pairs (IPSD₂). IPSD₁ from ts4 was more heterogeneous than that from wild-type (wt), but both contained sequences from all parts of the viral genome. IPSD₂ contained heterogeneous cellular sequences and viral sequences from the left 4.4% of the genome. An endonuclease activity associated with IP and virions was capable of digesting viral or cellular DNA to IPSD₂-like fragments suggesting a possible origin for these molecules.

Present address: Department of Biochemical Sciences, Princeton University, Princeton, New Jersey 08544, U.S.A.

Received 20 December 1980; accepted 23 January 1981.