HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Mamoun, R. Z. Articles by Guillemain, B. Search for Related Content PubMed PubMed Citation Articles by Mamoun, R. Z. Articles by Guillemain, B. Agricola Articles by Mamoun, R. Z. Articles by Guillemain, B.

Establishment and Propagation of a Bovine Leukaemia Virus-producing Cell Line Derived from the Leukocytes of a Leukaemic Cow

Institut National de la Santé et de la Recherche Médicale, Unité 117, 229, cours de l'Argonne, 33076 Bordeaux, France

A cell line (LB59Ly) derived from the leukocytes of a leukaemic cow was established as a monolayer, which spontaneously released large amounts of a retrovirus. This virus was found to be indistinguishable from the bovine leukaemia virus (BLV) produced by the commonly used high-producing heterologous cell line (FLK-BLV). Like the latter, its reverse transcriptase activity was greater in the presence of Mg²⁺ cations than in the presence of Mn²⁺ cations; its polyacrylamide gel electrophoresis pattern showed the presence of gp51, p24, p15, p12 and p10, and the antigenicity of the two major proteins completely cross-reacted with those of BLV from FLK-BLV cells. The virus was infectious and induced early and late polykaryocytosis, the specificity of which was demonstrated by use of specific anti-BLV sera.

Received 3 November 1980; accepted 23 January 1981.