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Cross-linking of the Subunits of Potato Virus X with Di-imidates

Rothamsted Experimental Station, Harpenden, Herts., U.K.

The protein subunits of the X^N strain of potato virus X (PVX) were cross-linked by di-imidates to produce a series of polymers in the proportion expected from the random cross-linking of subunits in an extensive heterologous array. Polymerization of the subunits of chemically modified forms of the virus, as well as of other strains, suggests that the cross-links are heterogeneous, and may, but need not, involve the two amino groups that have been previously characterized as either reacting with chlorogenoquinone or being sensitive to trypsin.

Received 12 December 1980; accepted 23 February 1981.