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J Gen Virol 54 (1981), 403-407; DOI 10.1099/0022-1317-54-2-403
© 1981 Society for General Microbiology
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Varicella-Zoster Virus Infection of Diploid and Chemically Transformed Guinea-pig Embryo Cells: Factors Influencing Virus Replication

Betty J. Edmond{dagger}, Charles Grose and Philip A. Brunell

Virology Laboratory Department of Pediatrics University of Texas Health Sciences Center, San Antonio, Texas 78284, U.S.A.

Factors influencing the replication of varicella-zoster virus (VZV) in guinea-pig embryo cells were evaluated using both diploid cells (GPEC) and a chemically transformed cell line (GPT). Wild-type and vaccine strains of VZV were successfully isolated and serially propagated in GPEC prepared from early gestation whole embryos (<2 cm in length). Low passage GPEC (≤5 subcultivations) were more susceptible to VZV infection than high passage GPEC (>5 subcultivations), and guinea-pig cells were consistently less permissive than human diploid cells. Cell-free virus was produced from VZV-infected GPEC cultures by sonication and peak yields of 103 p.f.u./ml were obtained. In addition, we report the isolation and propagation of VZV, as well as production of cell-free virus, in GPT. Both GPEC and GPT cells were less susceptible to VZV infection than human cells. However, viral replication was enhanced by incubation of VZV-infected GPT cultures at 32 °C rather than 36 °C.

{dagger} Present address: Department of Pediatrics, University of Iowa, Iowa City, Iowa 52242, U.S.A.

Received 20 June 1980; accepted 29 January 1981.




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Copyright © 1981 by the Society for General Microbiology.