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Transcription and Translation of the Herpes Simplex Virus Type 1 Thymidine Kinase Gene after Microinjection into Xenopus laevis Oocytes

MRC Virology Unit, Church Street Glasgow G11 5JR, U.K.

The hybrid plasmid pTK1 consists of the herpes simplex virus type 1 (HSV-1) BamHI p fragment, which contains the thymidine kinase (TK) gene, inserted into the vector pAT 153. When pTK1 DNA was microinjected into nuclei of Xenopus laevis oocytes, functional HSV-1-specific TK was produced, showing that transcription and translation of the gene occurred. Investigation of pTK1-specific RNA by ‘Southern’ blot hybridization revealed that all regions of the hybrid plasmid were transcribed by RNA polymerase II, but sequences present in TK mRNA were most highly represented in stable transcripts.

Received 24 November 1980; accepted 10 February 1981.