J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 54 (1981), 425-429; DOI 10.1099/0022-1317-54-2-425
© 1981 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tikchonenko, T. I.
Right arrow Articles by Naroditsky, B. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tikchonenko, T. I.
Right arrow Articles by Naroditsky, B. S.
Agricola
Right arrow Articles by Tikchonenko, T. I.
Right arrow Articles by Naroditsky, B. S.

The Distribution of Guanine-Cytosine Pairs in Adenovirus DNAs

T. I. Tikchonenko1, A. G. Dubichev1, Yu. L. Lyubchenko2, N. P. Kvitko2, N. M. Chaplygina1, T. I. Kalinina1, R. S. Dreizin1 and B. S. Naroditsky1

1 D.I. Ivanovsky Institute of Virology U.S.S.R. Academy of Medical Sciences
2 Institute of Molecular Genetics U.S.S.R. Academy of Sciences, Moscow, U.S.S.R.

The distribution of guanine-cytosine (GC) pairs in the DNA of the highly oncogenic simian adenovirus type 7 (SA7) and the non-oncogenic human adenovirus type 6 (Ad6) has been studied by thermal denaturation and CsCl density-gradient centrifugation. The differential of the DNA thermal denaturation curves shows the presence of pronounced peaks which indicates uneven distribution of GC pairs along the DNA chains and the presence of regions with GC content from 30 to 74% in SA7 DNA and from 40 to 68% in Ad6 DNA. The DNA restriction fragments obtained by treatment with EcoRI, BamHI, SalI, BglII and HindIII were subjected to CsCl density-gradient centrifugation. GC content of the fragments ranged from 45 to 70% for SA7 DNA and from 43 to 61% for Ad6 DNA. The GC content of the extreme left-hand fragments, where the transforming gene(s) is located, was higher than the average for SA7 DNA and lower than the average for Ad6 DNA. The most GC-rich regions were localized in the centre of the genome. The GC content of the right-hand part of both viral genomes was lower than the average.

Received 4 August 1980; accepted 30 January 1981.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1981 by the Society for General Microbiology.