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Host Restriction Property of a Vesicular Stomatitis Virus Mutant Isolated from Carrier Cultures

Timothy R. Winship

Department of Microbiology, School of Medicine State University of New York at Buffalo, Buffalo, New York 14214, U.S.A.

Carrier cultures of L cells infected with wild-type vesicular stomatitis virus (VSV_o) were initiated without the use of defective-interfering particles or homologous interferon. The cloned viruses recovered from such carrier cultures after passage 21 were characterized as temperature-sensitive. Furthermore, these clones of the mutant showed restricted replication at permissive temperature in HEp-2 cell cultures as compared to the wild-type VSV_o. This restrictive replication of the mutant in HEp-2 cells was not due to a defect in the expression of virion-associated primary transcriptase activity in vivo, but due to the marked reduction in virus-specific amplified RNA synthesis. These results suggest that a host function(s) yet to be characterized may govern the synthesis of mutant virus-specific amplified RNA in HEp-2 cells.

Present address: Virology Laboratory, V.A. Medical Center, West Haven, Connecticut 06516, U.S.A.

Received 2 December 1980; accepted 10 February 1981.