HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sykora, K. W. Articles by Moelling, K. Search for Related Content PubMed PubMed Citation Articles by Sykora, K. W. Articles by Moelling, K. Agricola Articles by Sykora, K. W. Articles by Moelling, K.

Properties of the Avian Viral Protein p12

Max-Planck-Institut für Molekulare Genetik, D-1000 West Berlin-33, Federal Republic of Germany

The avian RNA tumour virus structural protein p12 was purified from avian myeloblastosis virus (AMV) by nucleic acid affinity chromatography to apparent homogeneity as judged from SDS-polyacrylamide gel electrophoresis. A filter-binding assay was used for the identification of p12. High concentrations of p12 precipitated nucleic acids out of solution in the absence of MgCl₂. Binding of p12 to single-stranded nucleic acids protected them from digestion with nucleases and resulted in a hyperchromic effect. These phenomena were reversible in the presence of salt. The affinity of p12 to nucleic acids was determined by competing for the binding of p12 to denatured radioactive DNA by various other nucleic acids. It was found that p12 bound preferentially to single-stranded nucleic acids and showed a higher affinity to poly(rI) than to poly(rC) and poly(rA). Purified RNA-dependent DNA polymerase activity from AMV was stimulated up to sixfold by p12, depending on the template. Solubilization of RNA in RNA-DNA hybrids by RNase H was inhibited in the presence of p12.

Keywords: avian myeloblastosis virus, p12 protein, SDS-PAGE

Received 17 November 1980; accepted 3 March 1981.

This article has been cited by other articles:

				G. Krishnamoorthy, B. Roques, J.-L. Darlix, and Y. Mely DNA condensation by the nucleocapsid protein of HIV-1: a mechanism ensuring DNA protection Nucleic Acids Res., September 15, 2003; 31(18): 5425 - 5432. [Abstract] [Full Text] [PDF]

				R. J. Fisher, A. Rein, M. Fivash, M. A. Urbaneja, J. R. Casas-Finet, M. Medaglia, and L. E. Henderson Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides J. Virol., March 1, 1998; 72(3): 1902 - 1909. [Abstract] [Full Text] [PDF]