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J Gen Virol 55 (1981), 469-473; DOI 10.1099/0022-1317-55-2-469
© 1981 Society for General Microbiology
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Temperature-sensitive HVJ (Sendai Virus) with Altered P Polypeptide Derived from Persistently Infected Cell Lines

Hisashi Ogura, Hiroshi Sato and Motoichi Hatano

Department of Virology, Cancer Research Institute Kanazawa University, 13-1 Takara-machi Kanazawa 920, Japan

HVJ isolated from culture fluids of G2, THEL and GM2 cells persistently infected with HVJ (G2-HVJ, THEL-HVJ and GM2-HVJ) were characterized in comparison with wild-type HVJ (HVJo). Viral structural proteins were analysed by 10% SDS-polyacrylamide gel electrophoresis and it was found that only the P polypeptides of all the HVJ clones isolated from G2-HVJ cells had a smaller size mol. wt. of 77000 (77K), than that of HVJo with a mol. wt. of 79000 (79K). One of six clones from THEL-HVJ cells and one of ten clones from GM2-HVJ cells exhibited the same migration pattern of P polypeptide as that of the clones from G2-HVJ cells. However, the other structural proteins were not different from those of the wild-type virions. All the clones from these carrier cultures were temperature-sensitive and were blocked in early step(s) required for RNA synthesis. These results indicate that some mutations(s) associated with P polypeptide could occur during the course of HVJ persistent infection in cell cultures.

Keywords: Sendai virus, ts mutant, altered P protein, persistent infection

Received 22 December 1980; accepted 13 April 1981.




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Copyright © 1981 by the Society for General Microbiology.