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2-Thiouracil Does Not Inhibit TMV Replication in Tobacco Protoplasts

Institute of Plant Physiology Biological Research Center Hungarian Academy of Sciences, H-6701 Szeged, P.O. Box 521, Hungary

2-Thiouracil (2TU) did not inhibit either the incorporation of ³²P into tobacco mosaic virus (TMV) RNA or virus multiplication in tobacco protoplasts. However, the specific infectivity of TMV extracted from 2TU-treated protoplasts was 10 to 20% of that of the virus extracted from untreated protoplasts. Using a fluorescent antibody technique we have demonstrated that up to 60 to 70% fewer cells became infected in 2TU-treated as compared to untreated half leaves. Therefore, 2TU may express its inhibitory effect in leaves by producing defective TMV particles which fail to spread the infection from cell to cell rather than by causing direct inhibition of virus multiplication.

Keywords: TMV, thiouracil, replication, inhibition

Received 20 January 1981; accepted 14 April 1981.