HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Petri, T. Articles by Dimmock, N. J. Search for Related Content PubMed PubMed Citation Articles by Petri, T. Articles by Dimmock, N. J. Agricola Articles by Petri, T. Articles by Dimmock, N. J.

Phosphorylation of Influenza Virus Nucleoprotein in vivo

Department of Biological Sciences University of Warwick, Coventry, CV4 7AL, U.K.

Two-dimensional analysis of polypeptides from A/FPV/Rostock/34 (FP/R)-infected chick embryo fibroblast cells using non-equilibrium pH gradient gel electrophoresis followed by polyacrylamide gel electrophoresis, showed that nucleoprotein (NP) was the only detectable virus phosphorprotein and was present in both the nucleus and cytoplasm. The kinetics of accumulation of phosphorylated NP in the nucleus and cytoplasm were similar, suggesting that the presence or absence of phosphate groups did not control the entry of NP into the nucleus. In the course of this study, two-dimensional analysis of [³⁵S]methionine-labelled FP/R-infected cells revealed some major differences from previously published work which are discussed.

Keywords: influenza A virus, nucleoprotein, protein phosphorylation

Received 19 March 1981; accepted 5 June 1981.

This article has been cited by other articles:

				E. K.-W. Hui and D. P. Nayak Role of G protein and protein kinase signalling in influenza virus budding in MDCK cells J. Gen. Virol., December 1, 2002; 83(12): 3055 - 3066. [Abstract] [Full Text] [PDF]

				S. K. Biswas, P. L. Boutz, and D. P. Nayak Influenza Virus Nucleoprotein Interacts with Influenza Virus Polymerase Proteins J. Virol., July 1, 1998; 72(7): 5493 - 5501. [Abstract] [Full Text] [PDF]