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Detection of Entomopoxvirus Proteins in Insect Cell Culture by Enzyme-linked Immunosorbent Assay (ELISA)

Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, New York 14853, U.S.A.

An indirect enzyme-linked immunosorbent assay (ELISA), using antibodies made against gradient-purified Amsacta moorei entomopoxvirus (EPV), detected down to 13 ng of virus protein. Little antigenic relatedness was detected by ELISA between the structural proteins of Amsacta EPV, Euxoa EPV, Melanoplus EPV and vaccinia virus. Antibodies made against Amsacta EPV occlusion body matrix protein cross-reacted extensively with the occlusion body protein of Euxoa EPV. A rapid increase in the biosynthesis of Amsacta EPV structural proteins in Estigmene acrea (BTI-EAA) cells was detected from 12 to 50 h after virus infection. Low concentrations of virus-specific proteins were detected by ELISA in extracts of Amsacta EPV-infected Trichoplusia ni (Tn-368) cells from 1 to 96 h after virus inoculation.

Keywords: ELISA, entomopoxvirus, structural proteins

Received 2 February 1981; accepted 23 June 1981.