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J Gen Virol 57 (1981), 245-250; DOI 10.1099/0022-1317-57-1-245
© 1981 Society for General Microbiology
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Analysis of Deoxycytidine (dC) Deaminase Activity in Herpes Simplex Virus-infected or HSV TK-transformed Cells: Association with Mycoplasma Contamination but Not with Virus Infection

Jean Charron and Yves Langelier{dagger}

Institut du Cancer de Montréal Centre Hospitalier Notre-Dame, Montréal, Université de Montréal, Montréal, Canada

Deoxycytidine (dC) deaminase activity has been previously reported to be induced in herpes simplex virus (HSV)-infected cells (Chan, 1977). In contrast, we report here that HSV infection of either hamster cells naturally deficient in this enzyme activity or mouse cells containing a low level of activity never resulted in appearance or stimulation of dC deaminase, whereas thymidine kinase (TK) was always induced. Surprisingly, dC deaminase activity, which differed by electrophoretic mobility from the mouse or human cell enzyme, was discovered in some cells selected for the presence of HSV TK after infection with u.v.-irradiated HSV. Evidence is presented which suggests that the appearance of this new enzyme was not due to the presence of virus genes but rather to mycoplasma contamination.

Keywords: HSV, dC deaminase, mycoplasma contamination, HSV TK transformation

{dagger} Present address: Institut du Cancer de Montréal, Centre Hospitalier Notre-Dame, 1560 Est, rue Sherbrooke, Montréal, P.Q., Canada H2L 4M1.

Received 19 March 1981; accepted 15 June 1981.




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Copyright © 1981 by the Society for General Microbiology.