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J Gen Virol 57 (1981), 407-413; DOI 10.1099/0022-1317-57-2-407
© 1981 Society for General Microbiology
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Evidence for the Involvement of Influenza A (Fowl Plague Rostock) Virus Protein P2 in ApG and mRNA Primed in vitro RNA Synthesis

Stuart T. Nichol{dagger}, Charles R. Penn and Brian W. J. Mahy

Division of Virology, Department of Pathology University of Cambridge, Laboratories Block Addenbrooke's Hospital, Cambridge, U.K.

Eleven temperature-sensitive (ts) mutants of influenza A (fowl plague, Rostock) virus were analysed for in vitro RNA transcriptase activity in reactions primed by ApG or globin mRNA at 31 °C or at 40.5 °C, the restrictive temperature for ts mutant growth. Only those ts mutants studied which were defective in RNA segment 1, coding for the virion P2 protein, were defective in RNA transcriptase activity when compared to wild-type virus. Mutants having a defect in the P2 protein had no significant RNA transcriptase activity in reactions at 40.5 °C primed by globin mRNA. However, one mutant showed RNA transcriptase activity similar to wild-type virus at 40.5 °C when ApG (0.3 mM) was used as primer. The results suggest that influenza (fowl plague, Rostock) P2 protein is directly involved in the mRNA priming reaction, as well as in the RNA transcription reaction in vitro.

Keywords: influenza A(FPV), ts mutant protein, RNA synthesis

{dagger} Present address: Department of Biology, University of California, San Diego, California 92093, U.S.A.

Received 12 May 1981; accepted 5 August 1981.




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Copyright © 1981 by the Society for General Microbiology.