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J Gen Virol 58 (1982), 13-23; DOI 10.1099/0022-1317-58-1-13
© 1982 Society for General Microbiology
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Comparison of the Oligosaccharide Structure of the Glycoprotein of Vesicular Stomatitis Virus and a Thermolabile Mutant (tl-17)

James S. Robertson1, James R. Etchison2 and Donald F. Summers3

1 Division of Virology, Department of Pathology, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 2QQ, U.K.
2 California Primate Research Center and Departments of Internal Medicine and Biological Chemistry, University of California, Davis, California 95616, U.S.A.
and3 Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, Utah 84132, U.S.A.

As a means of examining the extent to which the polypeptide structure of a virus glycoprotein contributes to the overall structure and composition of the carbohydrate moieties, we have made a detailed comparison of the structure of the oligosaccharide moieties of wild-type vesicular stomatitis virus (VSV) glycoprotein with those of a glycoprotein-defective mutant of VSV, tl-17 (VSV). Characterization of the oligosaccharides by ion-exchange and gel filtration chromatography after sequential enzymic degradation reveals similar structures in the wt and mutant glycoproteins. However, the altered polypeptide structure of the tl-17 glycoprotein affects the extent of addition of sialic acid and fucose, both of which are added late in the maturation of the glycoprotein.

Keywords: VSV(tl-17), glycoprotein, oligosaccharide structure

Received 11 May 1981; accepted 18 August 1981.




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