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J Gen Virol 58 (1982), 205-209; DOI 10.1099/0022-1317-58-1-205
© 1982 Society for General Microbiology
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Intracellular Nucleocapsid RNA of Mumps Virus

Micheline McCarthy1,2,{dagger} and Robert A. Lazzarini2

1 Department of Neurology Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, U.S.A.
2 Laboratory of Molecular Genetics National Institute of Neurological and Communicative Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20205, U.S.A.

Intracellular nucleocapsids, virtually free of host proteins and nucleic acids, were purified from cells acutely infected with several strains of mumps virus. Viral RNA obtained from the nucleocapsids was predominantly a single 50S species, with 28S RNA occurring as a secondary peak in the RNA profile. Nucleocapsids yielded approx. 9 µg 50S RNA per 109 infected cells. The 50S RNA was partially self-complementary, indicating that mumps viral nucleocapsids include full-length plus (+) sense antigenomes. Self-annealing and competition hybridization assays with 50S RNA suggest that 28S RNA includes duplexes consisting of full-length (+) and minus (-) sense 50S RNA. Properties of the viral RNA did not vary with the method of RNA isolation, time post-infection at which nucleocapsids were harvested, nor the strain of mumps virus.

Keywords: mumps virus, nucleocapsids, intracellular RNA

{dagger} Present address: Merck Sharp & Dohme Research Laboratories, West Point, Pennsylvania 19486, U.S.A.

Received 20 May 1981; accepted 26 August 1981.




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Copyright © 1982 by the Society for General Microbiology.