HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zeichhardt, H. Articles by Habermehl, K.-O. Search for Related Content PubMed PubMed Citation Articles by Zeichhardt, H. Articles by Habermehl, K.-O. Agricola Articles by Zeichhardt, H. Articles by Habermehl, K.-O.

Mouse Elberfeld (ME) Virus Determines the Cell Surface Alterations when Mixedly Infecting Poliovirus-infected Cells

Jörg R. Schlehofer

Hartmut Hampl

Institut für Klinische und Experimentelle Virologie, Freie Universität Berlin, Hindenburgdamm 27, D-1000 Berlin 45, Federal Republic of Germany

The surface alterations of HEp-2 cells induced by mixed infection with two different picornaviruses (poliovirus and ME virus) were compared by scanning electron microscopic and transmission electron microscopic studies and by ⁵¹Cr-release assay. The contribution of each of the viruses to the resulting surface changes was discernible, as investigations on the chronology of the cytopathic alterations demonstrated that the changes were distinct for either virus. The surface of ME virus-infected cells was characterized by large membranous structures (‘sheets’ and blebs) representing huge vacuoles. These sheets were not seen in poliovirus-infected cells. Poliovirus induced more prominent cell pycnosis, elongation of filopodia and condensation of collapsed microvilli on the cell surface than ME virus. Mixed infection with these two viruses led to surface alterations typical for ME virus. These ME virus-specific changes occurred irrespective of poliovirus reproduction or its inhibition by guanidine. ME virus-specific alterations also predominated in cytolytic membrane damage as expressed by ⁵¹Cr-release from infected cells. ⁵¹Cr-release was more pronounced from ME virus than from poliovirus-infected cells, even when ME virus reproduction was suppressed by interfering poliovirus. However, alteration of the internal structures of the infected cells was only dominated by ME virus when the reproduction of poliovirus was suppressed.

Keywords: picornaviruses, interference, cell membranes, guanidine

Present address: Institut für Virologie, Zentrum für Hygiene der Universität Freiburg, Hermann-Herder-Str. 11, D-7800 Freiburg i.Br., Federal Republic of Germany.

Present address: Department of Microbiology, Vanderbilt University, School of Medicine, Nashville, Tennessee 37232, U.S.A.

Received 5 May 1981; accepted 22 September 1981.