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J Gen Virol 58 (1982), 443-448; DOI 10.1099/0022-1317-58-2-443
© 1982 Society for General Microbiology
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Construction of a Hybrid Plasmid Molecule Containing the Total Coding Region of Semliki Forest Virus 26S mRNA

Claudia Kondor-Koch and Henrik Garoff

European Molecular Biology Laboratory, Postfach 102209, 6900 Heidelberg, Federal Republic of Germany

We have constructed a hybrid plasmid molecule that contains the complete coding sequences from the 26S mRNA of Semliki Forest virus. Five fragments which together covered the mRNA sequence were isolated from three original hybrid plasmids and joined together at five different restriction endonuclease cleavage sites using T4 DNA ligase.

Keywords: SFV, ligations, cDNA cloning, restriction enzyme fragments

Received 27 May 1981; accepted 16 September 1981.




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