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J Gen Virol 59 (1982), 111-129; DOI 10.1099/0022-1317-59-1-111
© 1982 Society for General Microbiology
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Use of Recombinant Plasmids to Investigate the Structure of the Human Cytomegalovirus Genome

J. D. Oram, R. G. Downing, A. Akrigg, A. A. Dollery, C. J. Duggleby, G. W. G. Wilkinson and P. J. Greenaway

Genetic Manipulation Laboratory, PHLS Centre for Applied Microbiology and Research, Porton Down, Salisbury, Wiltshire, SP4 OJG, U.K.

Human cytomegalovirus (HCMV) DNA was digested with restriction endonucleases and the fragments characterized with respect to molecular weight and relative mole proportions. The terminal fragments were identified by digesting HCMV DNA with exonucleases before restriction endonuclease treatment and subsequent gel analysis. The HindIII fragments of HCMV DNA were cloned in Escherichia coli and recombinant plasmids were characterized by digestion with restriction endonucleases and by molecular hybridization with HindIII, BglII and XbaI fragments of the virus genome. Data from these experiments were used to construct physical maps of HCMV DNA for the HindIII, BglII and XbaI restriction endonucleases. The terminal regions of the genome and the region containing fragment HindIII M were shown to be heterogeneous.

Keywords: cytomegalovirus, genome, cloning, mapping

Received 1 April 1981; accepted 19 October 1981.


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