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Potato Spindle Tuber Viroid-infected Tissues Contain RNA Complementary to the Entire Viroid

Aaron Zelcer^1,

¹ Department of Plant Pathology, Cornell University, Ithaca, New York 14853
and ² The Rockefeller University, New York, N.Y. 10021, U.S.A.

Potato spindle tuber viroid (PSTV) RNA, labelled in vitro with ¹²⁵I, was hybridized in solution to RNA prepared from uninfected and PSTV-infected Rutgers tomato plants and suspension cultures. Following hybridization to RNA from infected plants, ¹²⁵I-labelled PSTV was converted from its single-stranded form to double-stranded RNA; this conversion did not occur to a significant extent when the ¹²⁵I-labelled PSTV was incubated with RNA from uninfected tomato plants under identical conditions. Following fractionation of RNA from PSTV-infected tissue with 2 M-LiCl and chromatography on cellulose CF11 columns, the RNA species which hybridizes with the PSTV probe was found to be enriched in those fractions which are also enriched for double-stranded RNA. Fingerprint analysis of hybridized ¹²⁵I-labelled PSTV following recovery from the hybrids demonstrated that all regions of the viroid are represented in the complementary strands present in these RNA preparations.

Keywords: PSTV, viroid, complementary RNA

Present address: Department of Genetics, Volcani Research Center, Bet Dagan, Israel.

Received 23 July 1981; accepted 8 October 1981.

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