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Evidence that Potato Leafroll Virus RNA is Positive-stranded, is Linked to a Small Protein and Does Not Contain Polyadenylate

T. Tamada

Scottish Crop Research Institute Invergowrie, Dundee DD2 5DA, U.K.

RNA extracted from particles of potato leafroll virus (PLRV) infected tobacco mesophyll protoplasts. Treating the RNA with proteinase K did not abolish its infectivity. In messenger-dependent rabbit reticulocyte lysate, PLRV RNA induced the synthesis of specific polypeptides: a major product of mol. wt. 71000 but no product the size of coat protein. PLRV RNA is therefore positive-stranded. A genome-linked protein (apparent mol. wt. 7000) was detected in preparations of PLRV RNA but no polyadenylate sequence was found. These features may prove to be characteristic of luteoviruses.

Keywords: genome-linked protein, polyadenylate, potato leafroll virus, RNA

Present address: Hokkaido Central Agricultural Experiment Station, Naganuma, Hokkaido, Japan.

Received 28 July 1981; accepted 8 October 1981.

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