HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Alatossava, T. Search for Related Content PubMed PubMed Citation Articles by Alatossava, T. Agricola Articles by Alatossava, T.

Factors Affecting In vitro DNA Ejection of the Lactobacillus lactis Bacteriophage LL-H

Department of Genetics, University of Oulu, SF 90570 Oulu 57, Finland

Tris(hydroxymethyl)aminomethane (tris) inactivates Lactobacillus lactis bacteriophage LL-H in vitro. The inactivation is caused by DNA ejection. This effect occurs in tris-HCl buffer only. Both pH and temperature affect the sensitivity of the phage to the tris treatment. The divalent cation Mg²⁺ prevented the inactivating effect of tris at concentrations about 10³-fold lower than for the monovalent cation K⁺.

Keywords: Lactobacillus bacteriophage, phage inactivation, DNA ejection

Received 15 May 1981; accepted 9 October 1981.