J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 59 (1982), 39-45; DOI 10.1099/0022-1317-59-1-39
© 1982 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bino, T.
Right arrow Articles by Rosenberg, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bino, T.
Right arrow Articles by Rosenberg, H.
Agricola
Right arrow Articles by Bino, T.
Right arrow Articles by Rosenberg, H.

Involvement of the Kidney in Catabolism of Human Leukocyte Interferon

Tamar Bino1, Habib Edery1, Arieh Gertler2 and Hagai Rosenberg1

1 Israel Institute for Biological Research, Ness Ziona
and 2 The Hebrew University, Faculty of Agriculture, Rehovot, Israel

The metabolic fate of human leukocyte interferon (HuIFN-{alpha}) was studied after intravenous injection into rats and cynomolgus monkeys. At various intervals the animals were sacrificed and the HuIFN-{alpha} content determined in serum and various tissues. HuIFN-{alpha} quickly disappeared from the circulation and was found mainly in the kidneys, in which levels were at least 7- to 10-fold higher than in the liver, spleen, lungs, heart, brain and muscles. No interferon was detected in urine. Subcellular fractionation of kidney revealed that the mitochondrial-lysosomal fraction (15000 g) had a high HuIFN-{alpha} content. It was also found that HuIFN-{alpha} was rapidly inactivated by two types of proteinases found in the lysosomal fractions of rat, monkey and human kidneys, with an optimal pH of 3 to 4. The inactivation was partially inhibited by either pepstatin or leupeptin. Inactivation was totally prevented by a mixture of both inhibitors. Since it is known that interferon is scantily excreted in urine, our findings suggest that the kidney serves as a main site for its degradation.

Keywords: human leukocyte IFN, pharmacokinetics, kidney

Received 30 June 1981; accepted 30 September 1981.


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
D.-s. Wang, S. Ohdo, S. Koyanagi, H. Takane, H. Aramaki, E. Yukawa, and S. Higuchi
Effect of Dosing Schedule on Pharmacokinetics of Alpha Interferon and Anti-Alpha Interferon Neutralizing Antibody in Mice
Antimicrob. Agents Chemother., January 1, 2001; 45(1): 176 - 180.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. Ohdo, D.-S. Wang, S. Koyanagi, H. Takane, K. Inoue, H. Aramaki, E. Yukawa, and S. Higuchi
Basis for Dosing Time-Dependent Changes in the Antiviral Activity of Interferon-alpha in Mice
J. Pharmacol. Exp. Ther., August 1, 2000; 294(2): 488 - 493.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
S. Koyanagi, S. Ohdo, E. Yukawa, and S. Higuchi
Chronopharmacological Study of Interferon-alpha in Mice
J. Pharmacol. Exp. Ther., October 1, 1997; 283(1): 259 - 264.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1982 by the Society for General Microbiology.