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Studies on Proteins of Simian Sarcoma-associated Virus with Different Growth History

Laboratory of Pathology, State University of Leiden, Wassenaarseweg 62, 2333 AL Leiden, The Netherlands

Simian sarcoma-associated virus (SSaV) was repeatedly passaged on three human cell lines. The proteins of the progeny virus were analysed for the presence of variant polypeptides. Occasionally, a few variant polypeptides were observed. One-dimensional peptide maps of the major virus protein p30 revealed no modifications after 25 cycles of infection on the three cell lines studied. The peptide map of Pr65^gag of virus grown through 25 passages on a human chondrosarcoma cell line was slightly different from that of the virus stock before passaging. The relative amount of the virus protein p30 as compared to p18 and p16 (possibly the SSaV equivalents of p15E and p12E) was variable depending on the host cell. Virus grown on Daudi cells was relatively deficient in p18 and p16. These virus particles were morphologically altered and had a low infectivity.

Keywords: mutants, deficient virions, constant p30

Received 8 June 1981; accepted 7 October 1981.