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J Gen Virol 59 (1982), 273-282; DOI 10.1099/0022-1317-59-2-273
© 1982 Society for General Microbiology
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Demonstration of Interference between Dengue Virus Types in Cultured Mosquito Cells using Monoclonal Antibody Probes

D. Dittmar, A. Castro and H. Haines

Departments of Pathology and Microbiology, University of Miami School of Medicine, P.O. Box 016960 (R-37), Miami, Florida 33101, U.S.A.

Cultured Aedes albopictus cells (clone C6/36), persistently infected (PI) with dengue virus type 1 (dengue-1) were found resistant to superinfection with dengue virus type 3 (dengue-3). This was determined by indirect immunofluorescent (IF) staining of cultures using monoclonal antibody against a dengue-3 type-specific antigen. Dengue-1 PI cultures stained with this antibody 3 days after superinfection with dengue-3 virus (m.o.i. of 2) had dengue-3 antigen in 0.1 to 1.0% of the cells. Control cultures infected with dengue-3 at the same multiplicity contained dengue-3 antigen in greater than 90% of the cells. The resistance to superinfection was not interferon-mediated, and occurred within 20 h after primary infection. In cultures simultaneously infected with two dengue virus types, one virus type was excluded from replication in most cells. A small population of cells was also found (about 1%) that contained type-specific antigen of both dengue virus types.

Keywords: dengue virus, interference, monoclonal antibody, mosquito cells

Received 20 July 1981; accepted 11 November 1981.


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