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Specific Cross-linking of Capsid Proteins to Virus RNA by Ultraviolet Irradiation of Poliovirus

Institut für Klinische und Experimentelle Virologie der Freien Universität Berlin, Hindenburgdamm 27, 1000 Berlin 45, Federal Republic of Germany

Poliovirus was irradiated with u.v. light under conditions causing approx. 5% cross–linking of capsid protein to virus RNA. Cross-linked RNA–protein complexes, freed from unbound protein, were treated with nuclease, and then analysed on SDS–polyacrylamide gels. The smallest capsid polypeptide VP4 was found to be associated with the RNA to the greatest degree, followed by VP2 and VP1, while VP3 was attached only in trace amounts. Low radiation doses, which produced cross–linking of RNA to protein, did not cause breakdown of the virus particles or conformational changes of the capsid as examined physically and serologically. However, higher doses caused structural alterations of the virus capsid.

Keywords: poliovirus, RNA-protein complexes, u.v. irradiation, cross-linking

Received 13 October 1981; accepted 23 November 1981.

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