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J Gen Virol 59 (1982), 415-419; DOI 10.1099/0022-1317-59-2-415
© 1982 Society for General Microbiology
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In vitro Stimulation of Rabbit T Lymphocytes by Cells Expressing Herpes Simplex Antigens

A. K. Kapoor{dagger}, N. R. Ling{ddagger}, A. A. Nash§, A. Buchan|| and P. Wildy§

Departments of Experimental Pathology and Virology The Medical School, Birmingham B15 2TJ, U.K.

Lymphocyte stimulation responses to herpes antigens were studied using virus-infected X-irradiated cells. Rabbits were immunized with herpes simplex virus type 1 (strain HFEM) grown in RK13 cells. For in vitro stimulation assay BHK21 cells were X-irradiated (15000 rad) and infected with a high m.o.i. of a temperature-sensitive (ts) mutant (N102) of HFEM strain at the non-permissive temperature (38.5 °C) of virus. Virus antigens were expressed on the infected cells and there was no leakage of infectious virus into the medium at 38.5 °C. T lymphocytes from rabbits immunized with herpes simplex virus were specifically activated by herpesvirus-infected X-irradiated cells; lymph node cells from rabbits immunized with RK13 cells and from non-immune rabbits showed no proliferative response.

Keywords: HSV, rabbit, T cell stimulation, infected cells

{dagger} Present address: Department of Pathology, K.G.'s Medical College, Lucknow, U.P., India.

{ddagger} Present address: Department of Immunology, The Medical School, Birmingham B15 2TJ, U.K.

§ Present address: Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, U.K.

|| Present address: Department of Medical Microbiology, The Medical School, Birmingham B15 2TJ, U.K.

Received 2 October 1981; accepted 14 December 1981.




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Copyright © 1982 by the Society for General Microbiology.