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A Comparison of Adenovirus 12 Induced T and Tumour Antigens by Rate-zonal Centrifugation

Department of Medical Microbiology, University of Sheffield, and Virus Research Laboratory, Lodge Moor Hospital, Sheffield, England

The sedimentation properties of adenovirus 12 induced antigens were studied by rate-zonal centrifugation in linear sucrose gradients. Adenovirus 12 hexon and fibre antigens were estimated to have molecular weights of 22–25 x 10⁴ and 6–7 x 10⁴ daltons, respectively. In addition, two species of T antigen were separated by rate-zonal centrifugation. The two T antigen species had molecular weights of 8–9 x 10⁴ and 4–5 x 10⁴ daltons and were distinct from adenovirus 12 hexon and fibre antigens. Results were identical whether the adenovirus 12 antigens were extracted from infected H.Ep-2 or HEK cells. Similar studies of tumour antigen from transplanted adenovirus 12 induced tumours in hamsters and CBA mice, and two hamster cell lines grown in vitro, revealed the presence of two species of tumour antigen. Molecular weight estimates for the two species of tumour antigen indicated that these antigens were similar in molecular weight to the T antigens. Differences in the relative proportions of the two species of antigen in tumour extracts and in T antigen extracts were found. Treatment of tumour extracts with sodium deoxycholate or ribonuclease did not alter the sedimentation properties of the tumour antigens.

Received 10 July 1969; accepted 29 August 1969.