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Infection of Chick Embryo Fibroblasts With Template Active RNA From Avian Myeloblastosis Virus

I. Hloánek^*,,

Institute of Experimental Biology and Genetics

J. íman and L. Vepek

Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, Prague 6

The effect of different doses of viral RNA extracted from avian myeloblastosis virus was tested on RIF-free chick embryo cells. The results showed that the number of cells with positive gs-antigen was probably related to the dose of RNA. The specificity of viral RNA action was shown by a fluorescent antibody method and by the use of ribonuclease.

^* Present address: Houghton Poultry Research Station, Houghton, Huntingdon, England.

Part of the work reported in this paper was undertaken during the tenure of an Eleanor Roosevelt International Cancer Fellowship of the American Cancer Society awarded by the International Union Against Cancer.

Received 8 August 1969; accepted 18 September 1969.