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Isolation and Properties of a Pseudomonas acidovorans Bacteriophage

Department of Microbiology, University of British Columbia, Vancouver 8, B.C., Canada

The lytic bacteriophage W-14 was isolated from sewage using Pseudomonas acidovorans no. 14 as host. The phage had an icosahedral head some 85 nm. in diameter and a contractile tail some 140 nm. long. W-14 formed plaques on only a few strains of P. acidovorans. The phage gave biphasic absorption kinetics, with an adsorption constant of 1.9 x 10^-9 ml./min. The latent period was 60 min. and the burst size was about 300. The burst size was dependent upon culture age. The k_u.v. for inactivation of W-14 was 4.35 min.^-1. P. acidovorans was shown to possess a potent photoreactivating system. The heat of inactivation of W-14 was calculated to be 75,700 cal./mole. The phage gave biphasic thermal inactivation kinetics at 55° and 60° but not at 65°. The phage mutated spontaneously to a different plaque type. This mutation affected the adsorptive properties, the thermal sensitivity and the burst size of the phage.

^* Present Address: Department of Biology, University of Calgary, Calgary, Alberta, Canada.

Received 17 July 1969; accepted 22 August 1969.