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Preparation and Characterization of Temperate, Non-inducible Bacteriophage P2 (host: Escherichia coli)

Department of Microbial Genetics, Karolinska Institutet, 104 01 Stockholm

P2 phage particles contained DNA (38%) and protein (62%), and were assigned a particle weight of 5.8 x 10⁷ daltons, based on the known molecular weight of P2 DNA. The extinction (1 cm.) of suspensions of 10¹¹ particles per ml. was 0.09 at 260 nm. wavelength. In purified preparations 20 to 50% of the particles present were not detected by biological assay. The preparations were heterogeneous in respect to heat stability. Even at relatively low temperatures, changes in light scattering accompanied heat inactivation.

Received 1 May 1969; accepted 4 September 1969.

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