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Fluorescence Assay of Foamy Virus

Department of Microbiology, The Queen's University of Belfast, Grosvenor Road, BT12 6BN, Northern Ireland

The optimal conditions for an immunofluorescence assay in HEp2 cells of a strain of foamy virus were investigated. Adsorption for 2 hr was followed by incubation at 37° for 65 hr. The validity of the assay was established and it was shown to be as sensitive as titration of cytopathic effect at limit dilution. The sensitivities of BHK 21, HEp2, RK 13 and vervet cells to foamy virus were compared and distribution of virus antigen in HEp 2 cells at various stages of infection were investigated.

Received 4 July 1969; accepted 5 October 1969.

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