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J Gen Virol 6 (1970), 373-380; DOI 10.1099/0022-1317-6-3-373
© 1970 Society for General Microbiology

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Ribonucleic Acid Content of Encephalomyocarditis Virus

A. T. H. Burness*

Virus Research Unit, Medical Research Council Laboratories, Woodmansterne Road, Carshalton, Surrey

The RNA content of encephalomyocarditis virus was found to be 31.7%, a figure obtained by combining results from nitrogen and phosphorus determinations which gave 32.0%, from extinction measurements at 260 nm. on perchloric acid hydrolysates which gave 31.3% and from orcinol determinations which gave 31.8%. Specific extinction coefficients obtained were EFormula at 260 nm. = 77.4 for the virus in 0.1 M-KCl + 0.02 M-phosphate buffer, pH 8.0, and EFormula at 260 nm. = 221 for its RNA in 0.1 M-tris + HCl, pH 7.2.

* Present address and reprints from: Sloan-Kettering Institute for Cancer Research, New York, N.Y. 10021.

Received 4 September 1969; accepted 27 October 1969.





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