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J Gen Virol 6 (1970), 381-393; DOI 10.1099/0022-1317-6-3-381
© 1970 Society for General Microbiology
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Particle Weight and other Biophysical Properties of Encephalomyocarditis Virus

A. T. H. Burness* and F. W. Clothier*

Virus Research Unit, Medical Research Council Laboratories, Carshalton, Surrey, England and Sloan-Kettering Institute for Cancer Research, New York, New York 10021, U.S.A.

A particle weight of 8.50 x 106 for encephalomyocarditis virus was derived by combining results from (a), sedimentation velocity measurements on highly purified preparations of virus (0.1 to 5 mg./ml.) in 0.1 M-KCl+0.02 M-phosphate buffer, pH 8.0, which gave an SFormula of 162.3 s, (b), differential sedimentation studies of virus in the same solvent but containing various ratios of D2O to H2O which gave a partial specific volume of 0.678 ml./g., with results from (c), analysis of boundary spreading during low speed centrifugation of dilute virus solutions which led to a diffusion coefficient D20,w of 1.44 x 10-7 cm.2/sec. Sedimentation equilibrium studies on similar virus preparations in the same solvent gave a particle weight of 8.52 x 106. This suggested a hydrated particle diameter for the virus of 29.8 nm. and a frictional ratio f/fo of 1.130 which was consistent with that of a hydrated sphere containing 0.29 g. water/g. dry virus.

* Present address: Sloan-Kettering Institute for Cancer Research, New York, N.Y. 10021.

Received 4 September 1969; accepted 27 October 1969.




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